13059_2017_1325_MOESM1_ESM.pdf (766.61 kB)

Additional file 1: Figure S1. of Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases

Download (766.61 kB)
journal contribution
posted on 11.10.2017 by Dingbo Zhang, Huawei Zhang, Tingdong Li, Kunling Chen, Jin-Long Qiu, Caixia Gao
Complete DNA sequences of the sgRNA expression constructs. Figure S2. Comparison of the on-target activities of WT SpCas9 and its variants. Figure S3. The relative expression levels of the sgRNAs produced from two types of sgRNA constructs. Table S1. Target sequences and oligos used to construct sgRNA expression vectors. Table S2. Indel frequencies revealed by deep amplicon sequencing. Table S3. Oligos used to construct vectors of sgRNA with mismatches. Table S4. PCR primers used in this study. Table S5. Second round PCR primers with barcodes for deep amplicon sequencing. (PDF 766 kb)


National Transgenic Science and Technology Program