Additional file 1: Figure S1. of Efficient differentiation of human embryonic stem cells to arterial and venous endothelial cells under feeder- and serum-free conditions

Immunocytochemical analysis of pluripotency status of hESCs cultured over Matrigel. Figure S2. Immunocytochemical analysis of pluripotency status of hESCs cultured over fibronectin. Figure S3. Time-course analysis of early differentiation of hESCs towards mesodermal lineage. Figure S4. Time-course analysis of early differentiation of hESCs towards endodermal lineage. Figure S5. Differentiation of H9-hESCs towards endothelial lineage. Figure S6. Effect of VEGF on hESC-derived endothelial cells under serum-free conditions. Figure S7. Survey of angiocrine secretory profile of arterial and venous ECs using angiogenesis antibody array. Table S1: Legend of coordinates for the human angiogenesis array. Table S2. Sequences of primers used for real-time RT-PCR. Table S3. List of antibodies used for flow cytometry. Table S4. List of antibodies used for immunocytochemistry. Supplementary Methods. (PDF 17154 kb)