12915_2022_1387_MOESM19_ESM.pdf (44.54 kB)
Additional file 19 of Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides
journal contributionposted on 2022-08-25, 07:11 authored by Nayeli Escudero Castelán, Dean C. Semmens, Luis Alfonso Yañez Guerra, Meet Zandawala, Mario dos Reis, Susan E. Slade, James H. Scrivens, Cleidiane G. Zampronio, Alexandra M. Jones, Olivier Mirabeau, Maurice R. Elphick
Additional file 19. Concentration-response graphs for A. rubens kisspeptin-type receptors (ArKPR2,4,5,10,11) that were not activated by any of the candidate ligands tested. Key: Green = ArKP1.1, Pink = ArKP1.2, Purple = ArKP2.2; Blue = ‘cocktail’ of neuropeptides derived from the L-type SALMFamide precursor (ArS1.1-7); Red = ‘cocktail’ of neuropeptides derived from the F-type SALMFamide precursor (ArS2.1-8). Each point represents mean values (± S.E.M.) from at least four independent experiments, with each experiment performed in triplicate. Luminescence is expressed as a percentage of the maximal response observed in each experiment. Triton X-100 (olive green), which triggers luminescence via receptor-independent mechanisms, was tested as positive control to check for cell viability and the response to Triton X-100 in each experiment was assigned as 100% luminescence. The source data for these experiments are provided in additional file 15.