Figure S15. The Slug protein was preferentially SUMOylated. The SUMOylated levels of the Snail and Slug proteins in the steady state. HEK293T cells were cotransfected with plasmids encoding Flag-tagged Snail or Slug and GFP-tagged SUMO-1. Then, the cells were used for immunoprecipitation with an anti-Flag antibody followed by immunoblotting with the indicated antibodies. β-actin was used as the internal control. The asterisk and arrowhead indicate SUMOylated Slug and non-modified Snail and Slug, respectively. (PDF 37 kb)