13046_2018_996_MOESM15_ESM.pdf (37.2 kB)
Additional file 15: of Hypoxia-induced Slug SUMOylation enhances lung cancer metastasis
journal contribution
posted on 2019-01-06, 05:00 authored by Pei-Fang Hung, Tse-Ming Hong, Che-Chang Chang, Chung-Lieh Hung, Yuan-Ling Hsu, Yih-Leong Chang, Chen-Tu Wu, Gee-Chen Chang, Nei-Li Chan, Sung-Liang Yu, Pan-Chyr Yang, Szu-Hua PanFigure S15. The Slug protein was preferentially SUMOylated. The SUMOylated levels of the Snail and Slug proteins in the steady state. HEK293T cells were cotransfected with plasmids encoding Flag-tagged Snail or Slug and GFP-tagged SUMO-1. Then, the cells were used for immunoprecipitation with an anti-Flag antibody followed by immunoblotting with the indicated antibodies. β-actin was used as the internal control. The asterisk and arrowhead indicate SUMOylated Slug and non-modified Snail and Slug, respectively. (PDF 37 kb)