12915_2022_1387_MOESM14_ESM.pdf (80.66 kB)
Additional file 14 of Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides
journal contributionposted on 2022-08-25, 07:11 authored by Nayeli Escudero Castelán, Dean C. Semmens, Luis Alfonso Yañez Guerra, Meet Zandawala, Mario dos Reis, Susan E. Slade, James H. Scrivens, Cleidiane G. Zampronio, Alexandra M. Jones, Olivier Mirabeau, Maurice R. Elphick
Additional file 14. Comparison of luminescence measurements of CHO-K1 cells transfected with the A. rubens kisspeptin-type receptors ArKPR1-11 at 35 seconds after exposure to candidate peptide ligands (10-5 M). Each bar represents mean values (± S.E.M.) from at least two independent experiments, with each experiment performed in triplicate. ArKP1.1 (green), ArKP1.2 (pink), ArKP2.2 (purple), ‘cocktail’ of L-type SALMFamide precursor derived peptides ArS1.1-7 (blue), ‘cocktail’ of F-type SALMFamide precursor derived peptides ArS2.1-8 (red) and assay media as a negative control (black). Triton X-100 (olive green), which triggers luminescence via receptor-independent mechanisms, was tested as a positive control to check for cell viability. The source data for these experiments are provided in additional file 15.