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Additional file 14 of H3K27 modifiers regulate lifespan in C. elegans in a context-dependent manner

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posted on 2021-03-26, 04:55 authored by Abigail R. R. Guillermo, Karolina Chocian, Gavriil Gavriilidis, Julien Vandamme, Anna Elisabetta Salcini, Jane Mellor, Alison Woollard
Additional file 14: Figure S6. daf-16 dependence of lifespan extension due to tissue-specific knockdown or overexpression of utx-1. A-B: Lifespan assays were performed on animals tissue-specifically overexpressing utx-1 in the intestine (A) or neurons (B) subjected to daf-16 RNAi. Overexpression of utx-1 gave a moderate lifespan extension in both of these utx-1 overexpressing strains subjected to daf-16 RNAi (p=0.0001 (***) intestinal; p=0.0002 (***) neuronal), suggesting partial daf-16 independence in each case. N2 and daf-16 (RNAi) controls are shared between experiments A and B (as the experiments were performed as a large set) although the graphs are separated for clarity. C-F: Lifespan assays were performed on wild type and daf-16(mu86) animals subjected to global (C) as well as epidermis (D), neuron (E) and intestine-specific (F) knockdown of utx-1. Knockdown of utx-1 in the wild type background in all cases caused lifespan extension, whereas this was largely abrogated in daf-16(mu86) mutants. EV= Empty Vector control (i.e. worms fed HT115 bacteria transformed with L4440 RNAi vector lacking a genomic insert). See Additional file 15: Table S9 for full statistical analysis of lifespan data, including repeats.

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Wellcome Trust Biotechnology and Biological Sciences Research Council

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