Additional file 12 of H3K27 modifiers regulate lifespan in C. elegans in a context-dependent manner

Additional file 12: Figure S5. Spatial expression of jmjd-3.2 and utx-1. A-B: Animals overexpressing a full length jmjd-3.2::GFP construct in wildtype (A) and jmjd-3.2(tm3121) mutants (B) were visualised by epifluorescence microscopy and showed spatial expression in a specific subset of neurons (white arrows). C-D: Animals overexpressing full length utx-1::GFP in wildtype (C) and utx-1(tm3118) mutant background (D) both showed ubiquitous expression (white arrows). E-H: The expression of demethylase dead jmjd-3.2 (E-F) and utx-1 (G-H) constructs showed a very similar pattern to the non-mutated constructs. I-J: Whole worm images of animals overexpressing utx-1::GFP (I) and utx-1DD::GFP (J) show ubiquitous expression of utx-1. Scale Bar (A-H) = 10μM; Scale Bar (I-J) = 20μM.