MOESM7 of Transcriptional induction of capsidiol synthesis genes by wounding can promote pathogen signal-induced capsidiol synthesis

Additional file 7: Figure S5. Expression of EAS is induced by three different methods of wounding. Leaves of the wild-type tobacco were wounded by three different methods. Hole, one or four small holes per a 1-cm diameter circle were made in the leaves by pricking with a 10-μl tip. Crush, leaves were held with forceps strongly. Disc, discs were excised from the leaves and floated on water. The samples were harvested at 9 h after wounding, and the transcript levels of EAS were quantified by RT-qPCR, and their levels were normalized to the levels of Actin2.