Additional file 6: of Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer

Figure S5. Examination of miR-146a-5p/Numb axis in expanded CT26-SDCSCs. (A) RT-qPCR of cellular miR-146a-5p expression. Data represent the mean ± S.D. **P<.01. (B) Immunoblots for showing expression of Numb and β-catenin. (C) RT-qPCR of cellular miR-146a-5p and Numb expression upon silencing miR-146a-5p. Zc, a shRNA control; Z146a, a shRNA targeting miR-146a-5p. (D) Spheroid-forming capacity upon silencing miR-146a-5p in CT26-SDCSCs. Data represent the mean ± S.D. ***P<.001. (PDF 242 kb)