Additional file 4: of The role of fibrinolysis inhibition in engineered vascular networks derived from endothelial cells and adipose-derived stem cells

Aprotinin does not impair network formation of endothelial cells in a 2D setup. CD31 staining of endothelial cells (either HUVEC or ECFC) reveals tube-like structures when co-cultured with ASC in a 2D-setup devoid of fibrin. Aprotinin (100 KIU/ml) has no effect on HUVEC 2D tube formation. Scale bar: 100 μm or 50 μm as indicated. (DOC 370 kb)