MOESM8 of Receptor kinase FERONIA regulates flowering time in Arabidopsis

Additional file 8: Figure S8. Isolation and characterization of the ralf1 mutant and RALF1-OX lines (A) Verification of the location of the T-DNA insertion described in SIGnAL (http://signal.salk.edu/cgi-bin/tdnaexpress). For the ATG start codon, the black boxes are exons, and the white boxes are the UTR. The exact sites of the T-DNA insertions (indicated by triangles) were mapped by PCR and DNA sequencing of the PCR products. (B) The T-DNA insert was present in the ralf1 mutant but not in the WT genomic DNA. (C) The relative mRNA levels of the RALF1 genes in the WT and eight different RALF1-OX lines. ACTIN2 was used as the internal control to calculate the relative mRNA levels. The experiments were performed at least three times with similar results. (D) Transgenic RALF1-OX lines were verified by PCR. WT plants were used as a negative control.