MOESM6 of Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process

Additional file 6: Figure S6. Percent (%) study of solubility, refolding and overall recovery yield of scFv protein. The IBs of scFv were solubilized mildly and strongly with the corresponding mild and strong denaturing agents. Completely denatured scFvs fusion with 6xHis, GST and MBP tags were further allowed for in vitro refolding. The recovery yields of soluble and refolded scFv proteins achieved by mild solubilization and denaturation-refolding method were converted into percent yields as per the quantity of initial pellet protein. Furthermore, overall percent yields of pure scFvs recovered by these two methods were calculated and compared with each other.