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MOESM4 of Measuring DNA content in live cells by fluorescence microscopy

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posted on 2018-09-04, 05:00 authored by Cecil Gomes, Michael Harman, Sara Centuori, Charles Wolgemuth, Jesse Martinez
Additional file 4: Figure S2. Calculating cellular ploidy in live cells using Hoechst 33342. A diagrammatical representation of H2B-GFP labeled cells progressing through mitosis (grey arrows) is shown. DNA ploidy can be calculated for each mitotic cell by summing the nuclear fluorescence of Hoechst 33342 in the nascent daughter cells. A diploid and tetraploid example is illustrated.

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National Cancer Institute

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    Cell Division

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    Live-cell microscopyHoechst 33342ImagingDNA content

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