MOESM4 of A novel gene from a secondary metabolism gene cluster is required for microsclerotia formation and virulence in Verticillium dahliae

Additional file 4: Figure S4. Impact of Nag1 silencing on cell wall integrity. a Colony morphology of WT, Nag1-S3 and Nag1-S6 after grown 10 days on CM or CM containing 1.2 M NaCl, 1.2 M KCl, 0.5 M MgSO4, 10 μg/mL CFW and 100 μg/mL Congo red. b The relative growth rate of WT, Nag1-S3 and Nag1-S6 in response to stress conditions. The % growth was determined by using radial growth of treated samples divided the radial growth of untreated controls (100). c The diagram shows the sizes of the inhibition zone after treated with 5 and 15% H2O2. The size of inhibition zone was measured at 2 dpi. Different letters in (b) and (c) indicate significant differences at P ≤ 0.05. (JPG 642 kb)