13068_2018_1133_MOESM2_ESM.jpg (126.71 kB)
MOESM2 of A Pseudomonas putida efflux pump acts on short-chain alcohols
figureposted on 2018-05-11, 05:00 authored by Georg Basler, Mitchell Thompson, Danielle Tullman-Ercek, Jay Keasling
Additional file 2: Figure S2. Cell-level GFP expression using the L-arabinose inducible PBAD promoter in Pseudomonas putida DOT-T1E. Cells carrying the pBbB8k-GFP plasmid were induced overnight without (brown), with 1 mM (red), 10 mM (purple), and 100 mM (green) L-arabinose. Cell-level fluorescence of GFP was measured using flow cytometry. The distributions indicate a homologous and quantitative increase of expression at the cell level.