13068_2018_1133_MOESM1_ESM.jpg (65.15 kB)
MOESM1 of A Pseudomonas putida efflux pump acts on short-chain alcohols
figureposted on 2018-05-11, 05:00 authored by Georg Basler, Mitchell Thompson, Danielle Tullman-Ercek, Jay Keasling
Additional file 1: Figure S1. Plasmid stability of pBbB8k-TtgABC in Pseudomonas putida DOT-T1E. The plasmid-carrying strain was grown for 5 consecutive days with 2 mM L-arabinose for inducing expression of TtgABC and without kanamycin. The medium was renewed once per day. Plasmid stability was determined by plating on kanamycin plates and comparison of viable cell numbers.