Additional file 9 of PA1 participates in the maintenance of blood–testis barrier integrity via cooperation with JUN in the Sertoli cells of mice

Additional file 9: Fig. S7. PA1 together with JUN regulates downstream gene expression. a) GO-Biological process analysis of the 184 overlapped genes identified in both downregulated genes of Amh-pa1−/− Sertoli cells and PA1 bound genes. Biological process of actin cytoskeleton organization, cell adhesion and transforming growth factor beta receptor signaling pathway were labeled with asterisks. b) Upstream transcription factor analysis of downregulated genes in Pa1-knockout MEFs. The identified top 1 transcription factor JUN was labeled with asterisk. c) The endogenous co-immunoprecipitation results of PA1 and JUN in TM4 cells. The proteins of TM4 cells were collected and immunoprecipitated using anti-PA1 antibody and the products were further used for immunoblot analysis with anti-PA1 and anti-JUN antibodies. The bands for PA1 and JUN were labeled with the arrows. d) Venn diagram showing the overlap of PA1 bound genes in Sertoli cells with JUN bound genes in MCF-7 cells [43]. e) GO-Molecular function analysis of the 626 overlapped genes identified in both PA1 bound genes in Sertoli cells and JUN bound genes in MCF-7 cells [43]. Molecular function of protein binding, actin binding and actin filament binding were labeled with asterisks. f) GO-Biological process analysis of the 626 overlapped genes identified in both PA1 bound genes in Sertoli cells and JUN bound genes in MCF-7 cells [43]. Biological process of cell adhesion was labeled with asterisks. g) KEGG pathway analysis of the 626 overlapped genes identified in both PA1 bound genes in Sertoli cells and JUN bound genes in MCF-7 cells. KEGG pathway of adherens junction and gap junction were labeled with asterisks.