Additional file 9 of An organoid model of colorectal circulating tumor cells with stem cell features, hybrid EMT state and distinctive therapy response profile
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posted on 2022-03-09, 04:17 authored by Maria Laura De Angelis, Federica Francescangeli, Chiara Nicolazzo, Michele Signore, Alessandro Giuliani, Lidia Colace, Alessandra Boe, Valentina Magri, Marta Baiocchi, Antonio Ciardi, Francesco Scarola, Massimo Spada, Filippo La Torre, Paola Gazzaniga, Mauro Biffoni, Ruggero De Maria, Ann ZeunerAdditional file 9: Figure S5. Selected protein validations on CTCDOs, XDOs, PDOs and CTCDOs/XDOs-derived xenografts. Left: immunoblot analysis of E-Cadherin, Vimentin, p21, P53, PON2, pHSP27 (Ser78), HIF-1α, Sirt2, Bcl-2, MMP2, Cathepsin D and Survivin on whole lysates of CTCDOs, XDOs, PDOs and CTCDOs/XDOs-derived xenografts (reported as CTCDOs xeno and XDOs xeno). β-actin and GAPDH were used as a loading control. Right: quantification of immunoblot experiments.
