40478_2020_965_MOESM7_ESM.tif (483.59 kB)
Additional file 7 of RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases
figureposted on 2020-06-26, 03:53 authored by Mahlon Collins, Yang Li, Robert Bowser
Additional file 7: Figure S7. Quantitative immunocytochemical analysis of RBM45 and SAFB during acute and chronic stress conditions. Nuclear RBM45 and SAFB were quantified by immunocytochemistry and image analysis in untreated and acute and chronically stressed HEK293 cells. To compare RBM45 and SAFB on the same scale, each protein’s signal was converted to Z scores. (a) Nuclear RBM45 levels in untreated and stressed cells. The genotoxic stressor mitoxantrone (MTX, acute = 6 h, 5 μM; chronic = 24 h, 1 μM), the heavy metal stressor cadmium sulfate (CdSO4, acute = 2 h, 30 μM; chronic = 24 h, 5 μM), and the oxidative stressor sodium arsenite (Ars, acute = 1 h, 1 mM; chronic = 24 h, 0.1 mM) all significantly reduced the nuclear RBM45 signal compared to untreated cells. (b) Acute MTX treatment, but not chronic MTX treatment significantly reduced the nuclear SAFB signal compared to untreated cells. Acute and chronic CdSO4 treatment significantly reduced the nuclear SAFB signal compared to untreated cells. Acute sodium arsenite treatment, but not chronic sodium arsentie treatment significantly reduced the nuclear SAFB signal compared to untreated cells. For (a) and (b), * = p < 1 × 10− 5, # = p < 0.01.