Additional file 7 of Long non-coding RNA NORAD promotes pancreatic cancer stem cell proliferation and self-renewal by blocking microRNA-202-5p-mediated ANP32E inhibition

Additional file 7: Figure S6. NORAD overexpression facilitates the self-renewal and stemness of PCSCs. A, Overexpression efficiency of NORAD verified by RT-qPCR in MIAPaCa-2 cells. B, ALDH activity of PCSCs assessed by Aldefluor assay, where mock means a NC with the addition of DEAB (a specific inhibitor of ALDH enzyme); C, Proliferation of PCSCs detected by MTT; D, Apoptosis and cell cycle changes of PCSCs measured by flow cytometry; E, Protein expression of PARP1 and the ratios of cleaved-caspase3 to pro-caspase3, and of cleaved-caspase9 to pro-caspase9 in PCSCs detected by Western blot analysis; F, Colony formation of PCSCs assessed by colony formation assay; G, Self-renewal ability of PCSCs detected by sphere formation assay (200 ×); H, Protein expression of Oct4, Nanog, Sox2 in PCSCs measured by Western blot analysis; * p < 0.05 vs. cells treated with NORAD-NC. Measurement data were expressed as mean ± standard derivation. Data among multiple groups were analyzed by one-way analysis of variance with Tukey's post hoc test, and data comparison among multiple groups at different time points was conducted using two-way analysis of variance with Bonferroni post hoc test. The experiment was repeated three times.