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Additional file 6 of Type I collagen facilitates safe and reliable expansion of human dental pulp stem cells in xenogeneic serum-free culture

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posted on 2020-07-14, 04:37 authored by Mai Mochizuki, Hiroshi Sagara, Taka Nakahara
Additional file 6: Figure S6. Flow cytometry of the reference samples. (A): Cells were dissociated from the confluent COL-XFM cultures on day 10 and the suspension of live cells were gated in the forward scatter (FSC-A) and side scatter (SSC-A) dot plots to eliminate cell debris and aggregates. (B): For JC-1 staining, unstained cells were used as a negative control to determine background levels and autofluorescence. Cells cultured for 7 days without media change were used as a positive control: damaged mitochondria were confirmed by decrease of JC-1 fluorescence aggregates (and increase of JC-1 monomers).

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Japan Society for the Promotion of Science Nippon Dental University

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