Additional file 6 of The Q/R editing site of AMPA receptor GluA2 subunit acts as an epigenetic switch regulating dendritic spines, neurodegeneration and cognitive deficits in Alzheimer’s disease

Additional file 6: Sup Figure 6. Genetically encoding GluA2(R) in J20 mice does not prevent neuroinflammation. (a) Representative images and stereological quantification revealed no changes were observed in the population of GFAP+ astrocytes within the CA1 and CA3 hippocampal regions (CA1 ANOVA F(3,16)  =  0.39 p = 0.76 and CA3 ANOVA F(3,16)  =  2.27 p = 0.12; n = 5/genotype). (b) Representative images and stereological quantification of CD68+ microglia in the hippocampus demonstrated a significant increase in both J20 and GluA2G/G/J20 animals when compared to WT and GluA2G/G mice (Kruskal-Wallis = 21.52 p <0.0001; n = 8 for WT and n = 7 for all other genotypes). (c) An ELISA revealed TNF protein expression in the hippocampus is significantly upregulated in J20 mice compared to WTs and this was not prevented in the GluA2G/G/J20 mice (ANOVA F(3,28)  =  8.65 p <0.001; n = 8/genotype). Each value represents the mean ± the SD. *p<0.05, **p<0.01, ***p<0.001.