Additional file 6: of TLR2 and TLR9 modulate enteric nervous system inflammatory responses to lipopolysaccharide

MCP-1 staining is more prominent in TLR4-expressing cells. ENS cultures were stimulated for 24 h with LPS and costained for MCP-1 and TLR4. Although cells displayed different staining intensities for TLR4, positive populations showed increased MCP-1 intensity (open arrows). MCP-1 was also observed in TLR4-negative cells, presumably macrophages, but the staining intensity was lower (white arrows). Scale bars: 25 μm. (TIF 857 kb)