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Additional file 6 of St13 protects against disordered acinar cell arachidonic acid pathway in chronic pancreatitis

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posted on 14.05.2022, 05:10 by Rong-chang Cao, Wan-jun Yang, Wang Xiao, Lei Zhou, Jie-hui Tan, Meng Wang, Zhi-tao Zhou, Huo-ji Chen, Jia Xu, Xue-mei Chen, Yang-chen Jin, Jia-yu Lin, Jun-ling Zeng, Shu-ji Li, Min Luo, Guo-dong Hu, Jin Jin, Xiao-bing Yang, Da Huo, Jie Zhou, Guo-wei Zhang
Additional file 6: Fig. S6. Parecoxib improves CP outcomes. (A) Molecular structures of parecoxib and valdecoxib. Parecoxib is metabolized to valdecoxib by the liver in vivo. (B) Representative immunohistochemical staining images of COX-2 in pancreatic tissues from humans and PRSS1Tg mice with CP. (C) The levels of IL-1β, IL-6, and TNF-α in the supernatants of valdecoxib-treated acinar cells from caerulein/EtOH-treated or untreated PRSS1Tg mice were measured by ELISA. (D, E) Micro-PET/CT was performed using 68Ga-DOTA-TATE or 68Ga-FAPI-04 to detect the pancreatic boundary and fibrosis in mice on day 28 after treated with caerulein or EtOH. (F, G) The tracer biodistribution in the lung, spleen, duodenum, muscle, pancreas, heart, liver, and kidney was calculated as the % ID/g of target organ to show tracer uptake in the parecoxib-treated and untreated nACP or ACP model animals.

Funding

National Natural Science Foundation of China Guangdong Science and Technology Planning Project Scientific Research Startup Program of Southern Medical University by High-level University Construction Funding of Guangdong Provincial Department of Education Clinical Research Program of Nanfang Hospital, Southern Medical University Clinical Research Startup Program of Southern Medical University by High-level University Construction Funding of Guangdong Provincial Department of Education

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