Additional file 6 of STOML2 potentiates metastasis of hepatocellular carcinoma by promoting PINK1-mediated mitophagy and regulates sensitivity to lenvatinib

Additional file 6: Figure S4. Lenvatinib induces cytoprotective mitophagy in HCC cells. (A) Double fluorescence staining of mitochondria by JC-1 was applied to monitor the mitochondrial membrane potential (MMP), as green fluorescent J-monomers indicating loss of MMP, and red fluorescent J-aggregates reflecting higher MMP. The MMP of SMMC-7721 and HCCLM3 with lenvatinib (10 μM, 4 h) treatment decreased significantly compared with controls by flow cytometric analysis. (B-C) Confocal microscopy was performed to detect spatial colocalization of mitochondrial protein COXIV (red) and PINK1 (green) (B) or TOMM20 (red) and LAMP1 (green) (Scale bars:10 μm) (C) in HCCLM3 cells with or without the treatment of lenvatinib (10 μM, 24 h). (Scale bars:10 μm) **P<0.01; ***P<0.001; ns, no significance.