Additional file 6 of Nucleoporin 153 deficiency in adult neural stem cells defines a pathological protein-network signature and defective neurogenesis in a mouse model of AD
Version 2 2024-12-06, 04:28
Version 1 2024-09-05, 09:39
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posted on 2024-12-06, 04:28 authored by Claudia Colussi, Alessia Bertozzi, Lucia Leone, Marco Rinaudo, Raimondo Sollazzo, Federica Conte, Elena Paccosi, Luca Nardella, Giuseppe Aceto, Domenica Donatella Li Puma, Cristian Ripoli, Maria Gabriella Vita, Camillo Marra, Marcello D’Ascenzo, Claudio GrassiSupplementary Figure 6: (A-B) Nitro-tyrosine (N-Tyr) and cysteine-S-nitrosylation (SNO-cys) levels evaluated by dot blot analysis (n = 3) in control, AD and AD-Nup organoids. Each lysate was obtained from the pool of 3–4 individual organoids. Hippocampal lysate from 9-month-old 3×Tg mice was used as positive control. Red ponceau (RP) staining was used as loading index and used to normalize samples. (C) Immunolabelling with Nav1.6 and Sox2 antibodies in control, AD and AD-Nup organoids and relative quantification of the mean fluorescence intensity (MFI) of the sodium channel Nav1.6. Nuclei were counterstained with DAPI. Scale bar 20 μm, *P < 0.05, ***P < 0.001
