Additional file 5 of The MYC/TXNIP axis mediates NCL-Suppressed CD8+T cell immune response in lung adenocarcinoma

Supplementary Material 5: Figure S5. The Effect of NCL on Cytotoxicity of CD8+ T Cells against CMT64 Cells. Note: (A) Flow cytometry analysis of tumor cell apoptosis, with apoptotic cells marked by red squares; the right panel shows the statistical analysis of apoptotic cells. (B) CCK-8 assay to assess tumor cell proliferation at different time points. (C) Confocal microscopy images displaying the infiltration of CFSE-labeled CD8+ T cells in MCS; scale bar=50 μm. The panel on the right shows the statistical analysis of the average CFSE fluorescence intensity (FL) in MCS. (D) Bright-field images of cell cultures captured in the inverted microscope mode, with white dashed lines outlining the invasive cell area; scale bar=50 μm. The right panel presents the statistical analysis of the invasive area. All cellular experiments were performed in triplicate, where * indicates P <, ** indicates P <, and *** indicates P <.