Additional file 5 of Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function

Additional file 5: Supplementary Figure 5. (A, B) Myogenic cells from mdx5cv and CD82-/-:mdx5cv mice were plated at the same density and induced to form myotubes. At day 5 following differentiation, cultures were immunostained for myosin heavy chain (red) to quantify the fusion index. (C) Quantification of fusion index shows significantly decreased fusion in CD82-/-:mdx5cv compared to mdx5cv cultures *p<0.05. (D) Quantification of non-adherent cells in mdx5cv and CD82-/-:mdx5cv cultures show no significant difference in their number up to 72 hrs following isolation.