Additional file 5 of PA1 participates in the maintenance of blood–testis barrier integrity via cooperation with JUN in the Sertoli cells of mice

Additional file 5: Fig. S3. Disorganized basal actin cytoskeleton is detected in Amh-pa1−/− mice testis. F-actin at the basal compartment of seminiferous tubules was observed to be perturbed and disorganized in adult Amh-pa1−/− testes. Arrows indicate F-actin bundles. Immunofluorescence using FITC-labeled phalloidin (green) was performed on the frozen testis sections of Pa1F/F mice (upper panels) and Amh-pa1−/− mice (lower panels). Nuclei were stained with DAPI (blue). Dashed boxes showed the localization of the enlarged images. Dashed curve indicated basal membrane of seminiferous tubules.