Additional file 5 of Nucleoporin 153 deficiency in adult neural stem cells defines a pathological protein-network signature and defective neurogenesis in a mouse model of AD

Supplementary Figure 5: (A-B) Western blot evaluation of chromatin regulators and histone modifications in control and AD iPSCs with the relative quantification. Optical density values of western blot data were calculated on the base of actin expression. (C) Representative images of Brd4 expression in control and AD iPSC at lower (scale bar 10 μm) and higher magnification (scale bar 5 μm; projections X-Y and Y-Z) and relative quantification of the mean fluorescence intensity (MFI). (D) Confocal analysis showing the level of Nup153 in iPSCs from control and AD samples with or without Nup153 transduction (n = 3, Scale bar 50 μm). Nuclei were counterstained with DAPI. E) Bright field images of embryoid bodies derived from control and AD-iPSCs transduced with GFP or Nup153-GFP (scale bar 100 μm). *P < 0.05, ** P < 0.01, *** P < 0.01