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Additional file 5 of Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast

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posted on 2020-07-11, 16:09 authored by William T. Molin, Allison Yaguchi, Mark Blenner, Christopher A. Saski
Additional file 5: Figure S3. Cloning of the eccDNA autonomous replication sequence in a yeast system. A. Water control (no colonies) B. pRS305 non-replicating plasmid (LEU marker replication − no colonies). C. pRS315 replicating plasmid (LEU marker, CEN6/ARS -lawn of colonies). D. pRS305 + CS-ARS1 + CEN6 (pRS305 + CEN6 + ARS1 − few colonies). E. pRS315 + CS-ARS1 + CEN6 (pRS315ΔARS + ARS1 − few colonies).