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Additional file 5: of Ancient role of vasopressin/oxytocin-type neuropeptides as regulators of feeding revealed in an echinoderm

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posted on 31.07.2019, 04:00 by Esther Odekunle, Dean Semmens, Nataly Martynyuk, Ana Tinoco, Abdullah Garewal, Radhika Patel, Liisa Blowes, Meet Zandawala, Jérôme Delroisse, Susan Slade, James Scrivens, Michaela Egertová, Maurice Elphick
Immunohistochemical assessment of the specificity of the asterotocin antiserum (A) Immunostaining in a transverse section of a radial nerve cord that was incubated with asterotocin antiserum (1:1000 dilution). Immunoreactive cell bodies (arrowheads) can be seen in the ectoneural epithelial layer and a dense network of stained fibres (asterisk) can be seen in the neuropile of the ectoneural region of the radial nerve cord. Staining can also be seen in the external epithelial layer of an adjacent tube foot (arrow). (B) Immunostaining in a transverse section of a radial nerve cord, adjacent to the section shown in (A), that was incubated with antiserum (1:1000) that had been pre-absorbed with the asterotocin antigen peptide (200 μM). Note that the majority of the immunostaining seen in (A) is absent in (B), but there is some residual staining in the ectoneural epithelial layer of the radial nerve cord (arrowheads) and in the epithelial layer of the adjacent tube foot (arrow). Therefore, antibodies to asterotocin were affinity-purified from the antiserum and used for the immunohistochemical analysis of asterotocin expression shown in Fig. 4. Abbreviations: Ec, ectoneural region; Hy, hyponeural region; TF, tube foot. Scale bars: (A) and (B) = 40 μm. (TIF 5965 kb)

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