Additional file 5 of Advances in the study of extracellular vesicles of Naegleria fowleri and their role in contact-independent pathogenic mechanisms

Additional file 5: Fig. S4. Effect of Nagleria fowleri extracellular vesicles (EVs) on the paracellular ionic permeability of MDCK cells. Transepithelial electrical resistance (TER) measurements were used to assess the ionic permeability of the paracellular pathway in the epithelium experimentally. Twenty-four hours after interaction, there was a significant decrease in the TER when MDCK cells interacted with N. fowleri EVs. These findings suggest that EVs increase paracellular ionic permeability. The data were analysed using one-way ANOVA followed by Tukey’s post hoc test. The error bars represent the standard deviation of the mean. Asterisks indicate significant differences between the experimental and control groups (*P < 0.05). n = 3.