Additional file 5: Figure S5. of Enhanced immunity in a mouse model of malignant glioma is mediated by a therapeutic ketogenic diet

Gating strategies for cytokine expression of CD8+ T cells. Tumor-infiltrating lymphocytes (TILs) isolated from gliomas from mice fed KD versus SD were cultured alone or in the presence of GL261-Luc2 tumor cells. Analysis of IFNγ (A), TNF (B) and IL-2 (C) production in tumor-infiltrating CD8+ T cells was performed. Percentage of positive cells indicated on plots. (TIF 9343 kb)