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Additional file 4 of Transcriptome analysis of a newly established mouse model of Toxoplasma gondii pneumonia
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posted on 2023-04-13, 13:06 authored by Long Cheng, Sajid Ur Rahman, Hai-Yan Gong, Rong-Sheng Mi, Yan Huang, Yan Zhang, Ju-Liang Qin, Cheng-Cong Yin, Min Qian, Zhao-Guo ChenAdditional file 4: Figure S1. Verification of transcriptome accuracy by qRT–PCR. The x-axis represents the DETs, and the y-axis represents the relative expression of the gene. DETs above the horizontal line are upregulated and those below the horizontal line are downregulated. qRT–PCR: quantitative real-time PCR; DETs: differentially expressed transcripts.
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Shanghai Agriculture Applied Technology Development Program Shanghai Science and Technology Commission Scientific Research Project National Key Research and Development Program of China Major Scientific Research Tasks of the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences Central Public-interest Scientific Institution Basal Research Fund National Data Center of Animal Health
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qrt – pcrdifferentially expressed transcriptslung tissue sectionslung pathology examinationlung morphological observationtranscript enrichment analysisevans blue stainingclinical cases due5 days postidentified 3167 detshost immune defensestranscriptome analysis helpedkegg enrichment analyses6 mice infectedresults provided detsnew mouse modelreported toxoplasma pneumoniagood disease modelgondii lung infectiontoxoplasma gondii infectiontranscriptome analysismice infectedlung tissuesmouse modeltoxoplasma gondiitoxoplasma pneumoniaclinical manifestations5 dpidets showeddets ).1880 detsinnate immuneimmune systemnasal infectiongondii rhtypical symptomssevere pneumoniaruffled furrh strainsresults c57blrespiratory cracklesquantitative realpulmonary edemaperformed usingpathological damagemolecular mechanismsmicrobial infectionsmediated signalingkyoto encyclopediainflammatory signalinggraphical abstractgene ontologye stainingdpi ).control groupbuffered salinebiological relevancebetter understand
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