Additional file 4 of The MYC/TXNIP axis mediates NCL-Suppressed CD8+T cell immune response in lung adenocarcinoma

Supplementary Material 4: Figure S4. Validation of NCL Silencing and Overexpression Efficiency. Notes: (A) Workflow for gene silencing/overexpression and cell grouping; (B-C) Assessment of NCL expression in cells post lentivirus transfection with shRNA using RT-qPCR (B) and Western Blot (C); (D-E) Evaluation of NCL expression in cells post lentivirus transfection with NCL plasmid using RT-qPCR (D) and Western Blot (E). All cellular experiments were replicated three times, with *** indicating statistical significance at P <.