Additional file 4 of Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function

Additional file 4: Supplementary Figure 4. CD82-/-:mdx5cv mice are more severely affected than mdx5cv at early age. (A) Representative image of mdx5cv and (B) CD82-/-:mdx5cv mice at 1 year of age. CD82-/-:mdx5cv mice show severe kyphosis. (C) Plethysmography assay shows significantly increased inspiration time in CD82-/-:mdx5cv compared to mdx5cv controls (***p<0.001). (D) Distribution plot of percentage of myofibers in CD82-/-:mdx5cv (red) and mdx5cv (blue) with minimum Feret diameter ranging from 10 to 90 microns. The plot shows how CD82-/-:mdx5cv mice have significantly smaller myofibers at 2 months of age. Comparisons were made with multiple t-tests (*=p≤0.05; **p≤0.01). (E) Serum creatine kinase (CK) activity assays were performed over a period of 20 weeks in independent cohorts of mice (n=4-8/timepoint/genotype). Multiple t-test analysis was made to compare the genotypes at each timepoint. The most significant changes in membrane permeability in CD82-/-:mdx5cv compared to control mdx5cv mice were observed between 5 and 10 weeks of age. (F-G) Examples of merged images of Sirius red stains used to quantify fibrotic tissue in mdx5cv and CD82-/-:mdx5cv mice at 2 months and one year. The images shown are examples of muscles at 2 months of age. Quantifications are shown in Fig. 4f.