Additional file 4 of Histone acetyltransferase NAA40 modulates acetyl-CoA levels and lipid synthesis

Additional file 4: Figure S4. Histone deacetylation-derived acetate is an important source for lipid accumulation upon NAA40 depletion. (A) Schematic representation of the different routes for the synthesis of acetyl-CoA in mammalian cells. (B) Representative immunoblot run in triplicate using antibodies against the indicated histone acetylation marks in scramble and NA40-KD cells with and without sodium butyrate, 48 h after siRNA treatment (n = 3/group). (C) Visualisation (left) and quantification (right) of lipid droplets by Nile red (red) and nuclei by DAPI (blue) in scramble and NAA40-KD cells with and without sodium butyrate, 48 h after siRNA treatment; Scale bar = 25 μm (n = 6-10/group). (D) RT-qPCR analysis of expression of Naa40, Acly and Acss2 mRNA levels in scramble and NAA40-KD, ACSS22-KD and NAA40-KD + ACSS2-KD cells after 48 h of siRNA treatment (n = 3/group). (E) Visualisation (left) and quantification (right) of lipid droplets by Nile red (red) and nuclei by DAPI (blue) in NAA40-KD, ACSS22-KD and NAA40-KD + ACSS2-KD cells after 48 h of siRNA treatment; Scale bar = 25 μm (n = 6-8/group). Data are presented as mean ± SEM and analysed by 1-way ANOVA with post hoc Dunnett’s multiple-comparisons test; *P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. Individual values can be found in Additional file 9: Table S10.