Springer Nature
13024_2022_561_MOESM4_ESM.tif (3.31 MB)
Download file

Additional file 4 of Farnesyltransferase inhibitor LNK-754 attenuates axonal dystrophy and reduces amyloid pathology in mice

Download (3.31 MB)
posted on 2022-08-21, 03:11 authored by Leah K. Cuddy, Alia O. Alia, Miranda A. Salvo, Sidhanth Chandra, Tom N. Grammatopoulos, Craig J. Justman, Peter T. Lansbury, Joseph R. Mazzulli, Robert Vassar
Additional file 4: Supplementary Fig. 4. Effect of LNK-754 and lonafarnib on brain levels of markers of farnesyltransferase inhibition. Immunoblot of brain homogenates from vehicle, LNK-754 and lonafarnib treated 5XFAD mice probed for prelamin-A (A), HDJ-2 (B), phospho-ERK1 (p-P44/P42) and total-P44/P42 (t-P44/P42) (C) and actin. Arrow denotes slower migrating HDJ-2 species in (B). Quantifications of prelamin-A (D) immunoblots in (A), HDJ-2 (*p = 0.036 for vehicle versus LNK-754, *p = 0.023 for LNK-754 versus lonafarnib) (E) immunoblots in (B) (upper band), p-P44/P42 (F) immunoblots in (C). Signals were normalized to actin and expressed as fold of vehicle. Triangles represent males and circles represent females. Vehicle, n = 11 (5 males, 6 females); LNK-754, n = 10 (4 males, 6 females); lonafarnib n = 10 (4 males, 6 females). 1-way ANOVA with Tukey’s post-hoc multiple comparisons test was performed.


National Institutes of Health