Additional file 4: of Amplified fragment length polymorphism and whole genome sequencing: a comparison of methods in the investigation of a nosocomial outbreak with vancomycin resistant enterococci

Figure S2. Phylogenetic tree of sequenced ST117 isolates. Included ST117 isolates are: 22 from the current outbreak, 4 recovered from the Netherlands between 2012 and 2015 (E8022, E8413, E7429 and E7486) and 1 isolate recovered from Sweden in 2014 (VRE1400294). The tree shows that H01 and S08 are a small sub-cluster within the outbreak cluster (Bootstrap value = 97%). It also shows how isolate S07, deemed part of the outbreak as per AFLP, is distinctly separated from the outbreak cluster. Isolate VRE1400294 from Sweden showed even closer phylogenetic relatedness to the outbreak cluster than S07. Isolates E8022, E87413, E7429 and E7486 come from four different cities across the Netherlands. The maximum likelihood tree is based on alignment of core-SNPs and is mid-point rooted. Red triangles indicate bootstrap values above 50% (size scaling proportionally from 50 to 100%). (PNG 47 kb)