Additional file 3 of Type I collagen facilitates safe and reliable expansion of human dental pulp stem cells in xenogeneic serum-free culture

Additional fil 3: Figure S3. Immunocytostaining of the HIF-1α localization in COL-XFM and XFM cells cultured in the presence of YC-1 under cobalt chloride-induced hypoxia conditions. Single cell analysis. Single channels (HIF-1α, DAPI, and vimentin) are shown in gray. The fluorescence intensities of HIF-1α (green) and nucleus (DAPI, magenta) are depicted by the dashed line (yellow) in a single cell, cell morphology was determined by vimentin (red), as shown in the merged images. Scale bars, 50 μm.