Additional file 3 of Roles of hsa-miR-12462 and SLC9A1 in acute myeloid leukemia

Additional file 3: Figure S2. The downstream targets of hsa-miR-12462. A. Differential expression of selected genes in hsa-miR-12462 OE and MOCK U937cells. B-C. (B) GO and (C) KEGG pathway enrichment analysis of differentially expressed genes after RNA-sequencing in U937 OE/MOCK cells. D-E. Transcript expression of CHRNA6 (D) and ARRB1 (E) in wild-type, MOCK-infected and OE U937 cells is shown. F-G. Luciferase reporter assay showing the 3'-UTR segments of (F) ARRB1 and (G) CHRNA6 do not contain hsa-miR-12462 binding sites. Error bars indicate ± SD; n=3 each. P values were obtained by the two-tailed Student t-test.