Additional file 3 of RNA dependent suppression of C9orf72 ALS/FTD associated neurodegeneration by Matrin-3

Levels of nuclear Histone H3 not altered in C9-ALS patient neurons. (A) Representative confocal images of C9-ALS iPSC differentiated to neurons (represented by MAP2, red) stained for Histone H3 nuclear protein (green/gray) (B) Quantification of endogenous Histone H3 immunofluorescence levels in MAP2+ neurons revealed no significant differences in the levels of nuclear Histone H3 in C9-ALS iPSC-MNs compared to that in control iPSC-MNs (Unpaired t test) n = 3 (C) Quantification of HIST1H3D mRNA fold change in control and C9-ALS iPSC MNs showed no significant differences in the mRNA levels in C9-ALS iPSC-MNs compared to that in control (Unpaired t-test) n = 10-12. Error bars indicate S.E.M