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Additional file 3 of Genome editing of CCR5 by AsCpf1 renders CD4+T cells resistance to HIV-1 infection

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posted on 09.07.2020 by Zhepeng Liu, Jin Liang, Shuliang Chen, Kewu Wang, Xianhao Liu, Beibei Liu, Yang Xia, Mingxiong Guo, Xiaoshi Zhang, Guihong Sun, Geng Tian
Additional file 3: Figure S2. CCR5 and CXCR4 editing simultaneously by CRISPR/AsCpf1 protects the cells from CXCR4-tropic and CCR5-tropic HIV-1 infection. A, the target sequence of screened CRISPR/AsCpf1 usingCXCR4-sgRNAs. B, T7E1 confirmed editing efficacy of the screened CXCR4-sgRNAs. C, DNA sequencing of CXCR4 fragment after CRISPR/AsCpf1-CXCR4-#2 editing. D, the simultaneously ablation efficacy of CXCR4 and CCR5 after co-delivery of CRISPR/AsCpf1-CXCR4-#2 and CRISPR/AsCpf1-CCR5-#4 into TZM.bl cells. E, the CXCR4 and CCR5 modified TZM.bl cells or control were challenged with R5-tropic HIV-1YU-2 and X4-tropic HIV-1NL4-3 mix (1: 1) at MOI = 0.5. The data shown were the mean ± SD of three independent experiments. **P < 0.01; NS, not significant; Statistical analysis determined using unpaired t-test.


China National Special Research Program of Synthetic Biology Shenzhen`s Health and Family Planning Commission Program of Clinic Research