Additional file 3 of Exosomes originating from infection with the cytoplasmic single-stranded RNA virus Rift Valley fever virus (RVFV) protect recipient cells by inducing RIG-I mediated IFN-B response that leads to activation of autophagy

Additional file 3: Figure S3. EXi-RVF Treatment Does Not Induce Apoptosis or Pyroptosis. A U937 cells were either left untreated or were treated with EXu or EXi-RVFV for 48 h, and caspase 1 activity was subsequently measured. Purified active human caspase 1 was included as positive control. For each treatment condition, mean values ± SEM from three biological replicates are shown. Statistical analysis was performed for comparison of purified caspase-1 treatment with untreated control. B U937 cells were either left untreated or were treated with EXu or EXi-RVFV for 48 h, and caspase 3 activity was subsequently measured. Irradiated U937 cells were included as positive control. For each treatment condition, mean values ± SEM from three biological replicates are shown. Statistical analysis was performed for comparing irradiated cells with untreated control. **P ≤ 0.01.