Additional file 3: of Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

Figure S3. A transposon insertion library of P. aeruginosa PAO1 generated with pSNC-mTn5. (a) Plasmid and transposon retention frequencies of the mTn5 insertion library of P. aeruginosa PAO1. (b) Colony restreaking assay. 100 random SucRKanR colonies were restreaked on LB + Kan and LB + Cam plates. 100/100 were found to be KanRCamS and 50 are shown here. (c) Colony PCR of ten restreaked clones in (b). All were found to be mTn5-positive and plasmid-negative. (d) mTn5 insertion sites of 37 mutant clones from the transposon insertion library of P. aeruginosa. Identical clones are only shown once, and their numbers are indicated in parenthesis. (TIF 1363 kb)