Additional file 3 of Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system

Additional file 3 Figure S3. Off-target editing. Off-target evaluation of RIG-I editing. a: The sequence of the top 2 off-target sites are represented along the gRNA sequence used for targeting RIG-I (red box in the middle). Blue letters indicate differences with the original sequence. An additional nucleotide was sequenced in the CHSE-EC cell line, not present in the published sequence (Otsh_v1.0, green box). b: Diagram representing the editing efficiency in the off-target regions (OffT1: ch7:73101728–73,102,398 and OffT2: ch14:42341999–42,342,859). No off-target was detected by Sanger sequencing in either sample (Puro- and Puro+; all sequences, including CHSE-EC (wt) were compared to CHSEwt). c: Representative chromatogram from the sequencing of off-target region 1 (OffT1) in CHSEwt (Control sample, bottom track) and CHSE-EC-RIG-I Puro + (Edited Sample, top track).