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Additional file 3 of Chorionic villus-derived mesenchymal stem cell-mediated autophagy promotes the proliferation and invasiveness of trophoblasts under hypoxia by activating the JAK2/STAT3 signalling pathway

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posted on 14.10.2021, 03:27 by Yijing Chu, Chengzhan Zhu, Chongyu Yue, Wei Peng, Weiping Chen, Guifang He, Changchang Liu, Yang Lv, Guoqiang Gao, Ke Yao, Rendong Han, Xiaoyu Hu, Yan Zhang, Yuanhua Ye
Additional file 3: Figure S3. Effect of hypoxia, IL-6 antibody and Baf A1 on trophoblasts treated with CV-MSC CM. (A) Three trophoblast cell lines were cultured under normal oxygen or hypoxic conditions, and p-JAK2, p-STAT3, BECN1, P62, LC3 and MMP2/9 expression levels was tested by western blotting analysis. (B) STAT3 in JEG-3 cells treated with cryptotanshinone at the indicated concentration or DMSO as a control for 60 min before culture in growth medium were tested by western blotting. (C) IL-6 antibodies were added to trophoblast cells treated with or without CV-MSC CM under hypoxic conditions, and p-JAK2, p-STAT3, BECN1, P62, LC3 and MMP2/9 expression levels were tested by western blotting analysis. Untreated trophoblast cells served as a control. (D) Baf A1 was added to trophoblast cells treated with or without CV-MSC CM under hypoxic conditions, and the expression of BECN1, P62 and LC3 was tested by western blotting analysis. Untreated trophoblast cells served as a control.

Funding

Clinical Medicine+X Project of the Affiliated Hospital of Qingdao University national natural science foundation of china young taishan scholars program of shandong province postdoctoral science foundation of china

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