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Additional file 3: Figure S3. of ROCK activity and the Gβγ complex mediate chemotactic migration of mouse bone marrow-derived stromal cells

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posted on 2015-07-24, 05:00 authored by Caroline Ryan, James Brown, Emer Bourke, Áine Prendergast, Claire Kavanagh, Zhonglin Liu, Peter Owens, Georgina Shaw, Walter Kolch, Timothy O’Brien, Frank Barry
ROCK inhibition affects morphology and MCP-1-induced migration in mouse BMSCs. Permeabilised serum-starved BMSCs immunofluorescently stained with F-actin (red) and α-tubulin (green) and DNA counter-stained with DAPI (blue). a Serum-starved BMSCs polarise in response to MCP-1; 10 μM Y27632 pre-treatment (24 h) results in a stellate morphology, with or without MCP-1 treatment. Scale bar = 10 μm. b Immunofluorescent images of representative fields of serum-starved (24 h) BMSCs pre-treated with 10 μM Y27632 and exposed to a gradient of MCP-1 in a 2D assay. Permeabilised serum-starved BMSC stained with F-actin (red) and α-tubulin (green) and DNA stained with DAPI (blue). Scale bar = 30 μm. 2D two-dimensional, BMSC bone marrow-derived stromal cell, DAPI 4′,6-diamidino-2-phenylindole, MCP-1 monocyte chemoattractant protein 1, ROCK Rho-associated, coiled-coil containing protein kinase. (PNG 1480 kb)