13024_2016_138_MOESM3_ESM.eps (25.59 MB)

Additional file 3: Figure S3. of Glymphatic distribution of CSF-derived apoE into brain is isoform specific and suppressed during sleep deprivation

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posted on 08.12.2016, 05:00 by Thiyagaragan Achariyar, Baoman Li, Weiguo Peng, Philip Verghese, Yang Shi, Evan McConnell, Abdellatif Benraiss, Tristan Kasper, Wei Song, Takahiro Takana, David Holtzman, Maiken Nedergaard, Rashid Deane
Radial distribution around arteries is greater for non-lipidated than lapidated apoE4. A-H) Representative images (from 3 to 4 mice) of radial diffusion of non-lipidated (A-D) and lipidated apoE4-Alexa647 that was colored green (E-H) at 10 min after their intracisternal injection in NG2ds-Red (NG2, red) reporter mice. The vasculature was outlined by intravascular labeling with lectin (colored blue). Scale bar = 100 μm. I) Clearance of 125I-apoE3 and 125I-apoE4 (10 nM) 90 min after their intracortical injection (0.5 μL over 5 min). J) FITC-apoE3 (10 nM) or 125I-apoE3 (10 nM) ISF clearance were similar 90 min after their intracortical injection. Values are mean ± SEM, n = 5-6 mice. (EPS 26205 kb)

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